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Metabolic profiling of Burkholderia cenocepacia, Burkholderia ambifaria, and Burkholderia pyrrocinia isolates from maize rhizosphere.

TitoloMetabolic profiling of Burkholderia cenocepacia, Burkholderia ambifaria, and Burkholderia pyrrocinia isolates from maize rhizosphere.
Tipo di pubblicazioneArticolo su Rivista peer-reviewed
Anno di Pubblicazione2005
AutoriAlisi, Chiara, Lasinio G.J., Dalmastri Claudia, Sprocati A.R., Tabacchioni Silvia, Bevivino Annamaria, and Chiarini L.
RivistaMicrobial ecology
Volume50
Paginazione385-395
ISSN00953628
Parole chiaveanalytical equipment, article, Burkholderia cepacia, classification, comparative study, Diagnostic, enzyme specificity, gardening, Maize, metabolism, microbiology, plant root, Plant Roots, Reagent Kits, Sensitivity and Specificity, species difference, Species Specificity, Substrate Specificity, Zea mays
Abstract

Burkholderia cenocepacia, Burkholderia ambifaria, and Burkholderia pyrrocinia are the Burkholderia cepacia complex (Bcc) species most frequently associated with roots of crop plants. To investigate the ecophysiological diversity of these species, metabolic profiling of maize rhizosphere isolates was carried out by means of the Biolog system, using GN2 and SFN2 plates and different parameters related to optical density (OD). The metabolic profiles produced by the SFN2 and GN2 plates were identical, but the SFN2's narrower range of OD values and significantly longer reaction times made these plates less suitable for differentiation of isolates. Principal component analysis of maximum OD (ODM) and maximum substrate oxidation rate (muM) data generated by GN2 plates allowed the selection of a reduced number of carbon sources. Statistical analysis of ODM values highlighted marked differences between the metabolic profiles of B. cenocepacia and B. ambifaria, whereas metabolic profiles of B. pyrrocinia clustered very often with those of B. cenocepacia. Analysis of the mu(M) parameter resulted in a slightly lower differentiation among the three Bcc species and a higher metabolic diversity within the single species, in particular within B. cenocepacia. Finally, B. cenocepacia and B. pyrrocinia showed generally higher oxidation rates than B. ambifaria on those GN2 substrates that commonly occur in maize root exudates.

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URLhttps://www.scopus.com/inward/record.uri?eid=2-s2.0-33645053027&doi=10.1007%2fs00248-005-0223-y&partnerID=40&md5=43bed61e78a2939b37d3e395d8384d07
DOI10.1007/s00248-005-0223-y
Citation KeyAlisi2005385